We looked at the distribution of strong and weak operon genes according to COG category and compared this to the overall distribution of COG categories in E. coli (Figure 8). Here r-protein genes were included. The strong operon genes are overrepresented in several of the COG categories compared to the weak operon genes; Translation, ribosomal structure and biogenesis (J), Transcription (K), Cell wall/membrane/envelope biogenesis (M), Energy production and conversion (C), Lipid transport and metabolism (I) and Secondary metabolites biosynthesis, transport and catabolism (Q). On the other hand, the weak operon genes are mainly overrepresented in Replication, recombination and repair (L), Posttranslational modification, protein turnover, chaperones (O) and Nucleotide transport and metabolism (F). This difference between strong and weak operon genes was confirmed with DAVID (excluding r-proteins), showing that whereas gene ontology terms like cell wall biogenesis and ATP metabolic process are overrepresented in strong operon genes, terms like DNA replication, response to stress and nucleotide binding are overrepresented in weak operon genes (p-values < 0.05 after Benjamini and Hochberg correction).
Strong and weak operon genes according to COG kinds. The newest chart includes ribosomal genes (Translation, ribosomal build and biogenesis (J)).
Adaptation in the evolutionary rate
Regarding the phylogenetic analysis i examined the entire evolutionary range centered on all family genes recognized as chronic. not, there will probably however become inter-gene version on the evolutionary price. This is analysed by using couples-wise Blast part results normalised up against alignment size; come across Approaches for further details.
Singleton in place of copy family genes
Earlier analyses have found a significant difference from the evolutionary speed from singletons and you may copies, but this image is actually strongly determined by the fresh 45 r-necessary protein in our investigation set. Analyses used having roentgen-proteins within the singletons group show that there’s indeed a distinction about your evolutionary rates. Brand new average of mediocre bit results (normalised more alignment length) try 0.81 to your singletons and you will 0.73 on copies (research maybe not found), implying that family genes for the groups reigned over by the singletons tend to be more similar to each other and you will evolve slower than just copies. But not, it is old-fashioned to depart out roentgen-necessary protein when examining evolutionary rates because they are very shown and you may evolve a great deal more more sluggish than many other https://datingranking.net/pl/datemyage-recenzja/ proteins. Without any roentgen-protein there can be zero significant difference amongst the singletons and you may duplicates (median regarding mediocre piece scores 0.71 and you can 0.72 respectively). Affirmed brand new roentgen-proteins develop slow which have a median from average part millions of 0.97. We as well as tested whether you will find people huge difference off protein duration for singletons and you can duplicates. Whenever roentgen-protein was indeed put aside, this analysis don’t provide people significant difference.
Strong as opposed to weakened operon family genes
I after that did an equivalent analyses just like the discussed more than, but contrasting solid and you can weak operon protein. The fresh new ribosomal together with fused/combined healthy protein have been put aside of your own investigation. The result is revealed into the Figure 9. The brand new median out of average piece scores to have good and you will weakened operon proteins is actually 0.65 and you will 0.79 correspondingly, ergo proving that strong operon family genes develop reduced compared to the weakened operon family genes (p-worthy of 3.527 ? 10 -5 ). Because the already mentioned the r-protein provides a median out-of mediocre section scores of 0.97. Additionally there is a difference out-of protein duration getting good and you may weak operon proteins. New protein of poor operon family genes (Shape 10) enjoys the typical period of proteins compared to amino acids getting proteins of strong operon genes (p-worth step 1.361 ? 10 -5 ).
Mediocre protein piece get having good and you will weakened operon gene groups. A box spot demonstrating different gene clusters ranked according to average couple-smart part rating of your necessary protein sequences (BitScore) normalised against positioning size (AliLen). Brand new legend text suggests the brand new median rating of each and every classification (weak operon 0.79 parts, good operon 0.65 pieces). Ribosomal genes commonly provided. If they are incorporated the newest numbers are 0.81 and you may 0.75, correspondingly.